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Image Search Results
Journal: Frontiers in Neuroscience
Article Title: An in Vivo Mouse Model to Investigate the Effect of Local Anesthetic Nanomedicines on Axonal Conduction and Excitability
doi: 10.3389/fnins.2018.00494
Figure Lengend Snippet: Experimental setup. (A) Tibial nerve fibers were stimulated proximally (PROX) at the sciatic notch of the ilium (sciatic nerve), to assess recovery of conduction across the local anesthesia (LA) site, and distally (DIST) to assess recovery of tibial nerve excitability. The evoked compound muscle action potential (CMAP) was recorded from the plantar muscles. (B) Needle electrode placement for PROX stimulation (S-, S+), CMAP recording (R1, R2), ground electrode (GND), and ECG recording (ECG-R, ECG-L); (C) Needle electrode placement for DIST stimulation (S-, S+), CMAP recording (R1, R2), and the ground electrode (GND). The detail at right depicts the local anesthetic injection needle (Inj) as well as the bubble engulfing the tibial nerve at the ankle. The baseline CMAP recordings evoked from the PROX and DIST stimulation sites were depicted in panels (D,E) , respectively. The amplitude CMAP was measured peak-to-peak (ppA) and the corresponding latency (Lat) to the first take-off from baseline. Note that from both stimulation sites, the evoked CMAP was followed by a secondary motor response (F-wave) resulting from antidromic activation of the motor neuron pool.
Article Snippet: The monitoring of the systemic toxicity was carried out using a
Techniques: Muscles, Injection, Activation Assay
Journal: Frontiers in Neuroscience
Article Title: An in Vivo Mouse Model to Investigate the Effect of Local Anesthetic Nanomedicines on Axonal Conduction and Excitability
doi: 10.3389/fnins.2018.00494
Figure Lengend Snippet: Development and recovery of conduction block after injection of the local anesthetic (LA). (A) The plantar compound muscle action potential CMAP evoked by proximal stimulation at the sciatic notch after non-encapsulated lidocaine (L. HCL) exposure. After 5 ms, the recordings are presented at 10-fold larger amplitude to facilitate the visualization of F-waves; (B) The relative changes in peak-to-peak CMAP amplitude (ppA) were shown for the non-encapsulated lidocaine (L. HCl) experiment in (A) versus 2 other different experiments using liposomal lidocaine (L. Lip); (C) ECG traces recorded during exposure to liposomal lidocaine. Note that the heart rate, measured from the QRS complexes, remained between 8 and 10 s (corresponding to 480–600 beats per minute). The ppA recovery corresponding to the ECG after 60 min is indicated in blue in panel (B) .
Article Snippet: The monitoring of the systemic toxicity was carried out using a
Techniques: Blocking Assay, Injection